Molecular Methodology
Microorganisms are detected and identified using the Polymerase Chain Reaction (PCR).
Since its inception, PCR has been used as an experimental tool for a multitude of experimental applications, including the identification of particular target sequences of DNA within specific organisms.
Each of the microorganisms that we test for involves the amplification of a specific target fragment of DNA that is unique to each microorganism. The amplification of a specific gene marker allows us to unequivocally detect and identify the presence or absence of a particular microorganism using PCR.
This technology significantly reduces false positive results and minimizes confirmation testing. This is a USDA/FSIS and AOAC approved technology.
In instances where additional confirmation of an organism is necessary, DNA sequencing and analysis may also be performed.
